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研究内容

生物は進化していく過程で、さまざまな環境に適用するために“多様”な機能を得てきました。例えば、視覚、味覚、臭覚などです。実は、色や味や匂いは、同じ分類に属する受容体（鍵穴）にそれぞれの化合物（鍵）が入ることによりシグナルが脳へと伝達され、それぞれの感覚として認識されることが知られています。このように、進化の過程で、ある一つの機能が”多様”に変化し、様々な機能へと分かれて行きました。我々の研究室では、ロマンチスト達が集まり「なぜ生物がこのように多様性に富んだ進化をしたのか？」という素朴な疑問に思いを馳せ研究してます。

主な研究テーマ

  1. 味覚修飾蛋白質（ミラクリン、ネオクリン）の味覚修飾のメカニズムの解明

    ある種の植物に含まれるミラクリンやネオクリンと呼ばれるタンパク質は酸っぱいものを甘く感じさせます。この不思議な効果をコンピュータシミュレーションと生化学的実験により解明し、新しい甘味タンパク質を創成する研究を行っています。

  2. うまみ受容体に働きうま味を増強させるうま味増強剤のデザイン

    日本食の特徴のひとつはうま味を有効に使い、適度な食事量で充分な満足感を得ることにあります。特にうま味の増強により過食を防ぐことが重要で、この増強のメカニズムについてうま味受容体とうま味物質の相互の働きの解析を行います。

  3. Gタンパク質共役型受容体に作用するステロイド誘導体のデザインと合成
    1. 匂い受容体に働き、匂いを変化させる分子のデザインと合成

      非常に多種類の匂い物質がわずか２５種類の匂い受容体によって感知されます。この多様な匂い物質の感知機構を解明するために、新規ステロイド誘導体をデザインし、実際に合成研究を行っています。

    2. 胆汁酸受容体TGR5に対する新規生理活性物質のデザインと合成

      TGR5アゴニスト活性を有する成分には、抗肥満、糖代謝改善効果が期待されることから、TGR5に対する新規リガンドの開発を目指し、研究を行っています。

  4. 関連受容体BR1に働く痛み防御物質のデザインと合成

    痛みに関連するペプチドであるブラジキニンの受容体BR1に働き痛みをブロックする天然物質とその構造に関連する化合物の合成を行っています。また、これらの構造と活性の相関の解析から、全く新しい鎮痛薬のデザインと合成を行っています。

  5. カルバ糖の合成と免疫化学への応用研究

    糖鎖の抗体は、主に腫瘍マーカーなどとして臨床の場で利用されています。しかしながら、ヒト型糖鎖は抗原となりにくいので糖鎖抗体を産生することが困難です。そのため、擬似糖鎖を利用した新しいヒト型糖鎖抗体作製法を研究しています。

主な使用機械

NMR, IR, PCR, 分子モデリングソフト

共同研究先

某有名企業、某有名国立大学（東大、京大）の多数と共同研究をしております。

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研究スタイル

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有機合成、生化学、コンピュータモデリング...........
全ての分野に専門家がいますが、それを繋げることの出来るBackgroundを持った人間はなかなかいません。我々の研究スタイルは、これら3つの分野の融合を目標にしております。あくまで、専門領域は有機化学ですが、これを一つのツール（道具）として用い、生物が進化の過程で獲得した機能の解明を行うことが目的です。

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論文

学術論文: 石黒正路 (2000年以降)

2015

  1. 150) Synthesis of the aglycon of pentalinonside, Nobuhisa Isaka, Saki Kawada, Masaji Ishiguro, and Minoru Tamiya, Heterocycles, 2015, 91, 1715–1721.

  2. 149) Structural Basis of pH Dependence of Neoclin, a Sweet Taste-Modifying Protein, Takayuki Ohkubo, Minoru Tamiya, Keiko Abe, Masaji Ishiguro, PLoS ONE (2015) 10 (5), e0126921.

2014

  1. 148) Akio Noguchi, Manabu Horikawa, Jun Murata, Masayuki Tera, Yosuke Kawai, Masaji Ishiguro, Toshiaki Umezawa, Masaharu Mizutani, Eiichiro Ono Mode-of-action and evolution of methylenedioxy bridge forming P450s in plant specialized metabolism. Plant Biotechnology (2014), 31(5), 493-503.

  2. 147) Total Synthesis of 2,6-Dideoxy-3-O-methylpyranosylillustrol, a Seco-norpregnaneglycoside from Mandevilla illustris, Tamiya, M.; Isaka, N.; Ishizawa, K.; Ikda, M.; Ishiguro, M. Chem. Lett. 43, 1704–1706.

  3. 146) Synthesis of the Aglycone of the Argelosides: Antiproliferative 14,15-seco-Pregnane Glycosides, Tamiya, Minoru; Isaka, Nobuhisa; Kitazawa, Takaki; Ishiguro, Masaji, Asian Journal of Organic Chemistry (2014), 3(3), 264-268.

2013

  1. 145) FFA1-selective agonistic activity based on docking simulation using FFA1 and GPR120 homology models, Takeuchi, Masato; Hirasawa, Akira; Hara, Takafumi; Kimura, Ikuo; Hirano, Tatsuya; Suzuki, Takayoshi; Miyata, Naoki; Awaji, Takeo; Ishiguro, Masaji; Tsujimoto, Gozoh, British Journal of Pharmacology (2013), 168(7), 1570-1583.

  2. 144) Reminiscences of research on the chemistry and biology of natural sterols in insects, plants and humans, Ikekawa, Nobuo; Fujimoto, Yoshinori; Ishiguro, Masaji, Proceedings of the Japan Academy, Series B: Physical and Biological Sciences (2013), 89(8), 349-369.

2012

  1. 143) A Concise Total Synthesis of the Non-peptide Bradykinin B1 Receptor Antagonist Velutinol A, Isaka, Nobuhisa; Tamiya, Minoru; Hasegawa, Atsushi; Ishiguro, Masaji, European Journal of Organic Chemistry (2012), 2012(4), 665-668.

  2. 142) Characterization of the modes of binding between human sweet taste receptor and low-molecular-weight sweet compounds, Masuda, Katsuyoshi; Koizumi, Ayako; Nakajima, Ken-ichiro; Tanaka, Takaharu; Abe, Keiko; Misaka, Takumi; Ishiguro, Masaji, PLoS ONE (2012), 7(4), e35380.

  3. 141) Chimeric yeast G-protein α subunit harboring a 37-residue C-terminal gustducin-specific sequence is functional in Saccharomyces cerevisiae, Hara, Keisuke; Inada, Yuko; Ono, Takuya; Kuroda, Kouichi; Yasuda-Kamatani, Yoshimi; Ishiguro, Masaji; Tanaka, Takaharu; Misaka, Takumi; Abe, Keiko; Ueda, Mitsuyoshi, Bioscience, Biotechnology, and Biochemistry (2012), 76(3), 512-516.

  4. 140) Mechanism of inverse agonist action of sarpogrelate at the constitutively active mutant of human 5-HT2A receptor revealed by molecular modeling, Hossain, Murad; Muntasir, Habib Abul; Ishiguro, Masaji; Bhuiyan, Mohiuddin Ahmed; Rashid, Mamunur; Sugihara, Takumichi; Nagatomo, Takafumi, Biological & Pharmaceutical Bulletin (2012), 35(9), 1553-1559.

2011

  1. 139) Modification of D-ring Moiety of Steroids–A Novel Palladium Catalyzed Baeyer–Villiger type Rearrangment of Cyclic Silylenol Ether Derivatives; Tamiya, M.; Takada, F.;Isaka, N.; Iimura, N.; Ishiguro. M. Heterocycle. 2011, 82, 1119–1125.

  2. 138) Metastable decomposition and hydrogen migration of ethane dication produced in an intense laser field; Hoshina, K.; Kawamura, H.; Tsuge, M.; Tamiya, M.; Ishiguro, M. J. Chem. Phys. 2011, 134, 064324-1–8.

  3. 137) Roles of Pseudomonas aeruginosa autoinducers and their degradation products, tetramic acids, in bacterial survival and behavior in ecological niches, Hosono Honda Natsue; Kimura Soichiro; Tateda Kazuhiro; Horikawa Manabu; Ueda Chihiro; Ishii Yoshikazu; Ishiguro Masaji; Miyairi Shinichi; Yamaguchi Keizo, Microbes and environments / JSME (2011), 26(2), 160-4.

  1. 136) Masuda K, Koizumi A, Misaka T, Hatanaka Y, Abe K, Tanaka T, Ishiguro M, Hashimoto M. Photoactive ligands probing the sweet taste receptor. Design and synthesis of highly potent diazirinyl D-phenylalanine derivatives.Bioorg Med Chem Lett. 20 1081-3 (2010).

  2. 135) Bhuiyan MA, Hossain M, Ishiguro M, Nakamura T, Nagatomo T. Engineered mutation of some important amino acids in angiotensin II type 1 (AT1) receptor increases the binding affinity of AT1-receptor antagonists. J Pharmacol Sci. 113 57-65 (2010).

  3. 134) Ito K, Sugawara T, Koizumi A, Nakajima KI, Shimizu-Ibuka A, Shiroishi M, Asada H, Yurugi-Kobayashi T, Shimamura T, Asakura T, Masuda K, Ishiguro M, Misaka T, Iwata S, Kobayashi T, Abe K. Bulky high-mannose-type N-glycan blocks the taste-modifying activity of miraculin. Biochim Biophys Acta. 1800 986-992 (2010)

  4. 133) Sakurai T, Misaka T, Ishiguro M, Masuda K, Sugawara T, Ito K, Kobayashi T, Matsuo S, Ishimaru Y, Asakura T, Abe K. Characterization of the {beta}-D-Glucopyranoside Binding Site of the Human Bitter Taste Receptor hTAS2R16. J Biol Chem. 285 28373-28378 (2010).

  5. 132) Sun Q, Hirasawa A, Hara T, Kimura I, Adachi T, Awaji T, Ishiguro M, Suzuki T, Miyata N, Tsujimoto G. Structure-activity relationships of GPR120 agonists based on a docking simulation. Mol Pharmacol. 2010 Aug 4. e-publication

  6. 131) Ono E, Homma Y, Horikawa M, Kunikane-Doi S, Imai H, Takahashi S, Kawai Y, Ishiguro M, Fukui Y, Nakayama T. Functional Differentiation of the Glycosyltransferases That Contribute to the Chemical Diversity of Bioactive Flavonol Glycosides in Grapevines (Vitis vinifera). Plant Cell. 2010 Aug 6. e-publication

  7. 130) Ueda C, Tateda K, Horikawa M, Kimura S, Ishii Y, Nomura K, Yamada K, Suematsu T, Inoue Y, Ishiguro M, Miyairi S, Yamaguchi K. Anti-Clostridium difficile Potential of Tetramic Acid Derivatives from Pseudomonas Quorum-Sensing Autoinducer. Antimicrob Agents Chemother.54 683-688 (2010)

2009

  1. 129) Tsuchihara K, Wazawa T, Ishii Y, Yanagida T, Nishida R, Zheng XG, Ishiguro M, Yoshihara K, Hisatomi O, Tokunaga F. Characterization of chemoreceptive protein binding to an oviposition stimulant using a fluorescent micro-binding assay in a butterfly. FEBS Lett. 583 345-349 (2009)

  2. 128) Kawasaki N, Lin CW, Inoue R, Khoo KH, Kawasaki N, Ma BY, Oka S, Ishiguro M, Sawada T, Ishida H, Hashimoto T, Kawasaki T. Highly fucosylated N-glycan ligands for mannan-binding protein expressed specifically on CD26 (DPPVI) isolated from a human colorectal carcinoma cell line, SW1116. Glycobiology. 19 437-450 (2009 )

  3. 127) Kimura S, Tateda K, Ishii Y, Horikawa M, Miyairi S, Gotoh N, Ishiguro M, Yamaguchi K. Pseudomonas aeruginosa Las quorum sensing autoinducer suppresses growth and biofilm production in Legionella species. Microbiology. 155 1934-1539 (2009)

  4. 126) Bhuiyan MA, Ishiguro M, Hossain M, Nakamura T, Ozaki M, Miura S, Nagatomo T. Binding sites of valsartan, candesartan and losartan with angiotensin II receptor 1 subtype by molecular modeling. Life Sci. 85 136-140 (2009)

  5. 125) Noguchi A, Horikawa M, Fukui Y, Fukuchi-Mizutani M, Iuchi-Okada A, Ishiguro M, Kiso Y, Nakayama T, Ono E. Local differentiation of sugar donor specificity of flavonoid glycosyltransferase in Lamiales. Plant Cell. 21 1556-1572 (2009)

  6. 124) Wakimoto T, Nitta M, Kasahara K, Chiba T, Ye Y, Tsuji K, Kan T, Nukaya H, Ishiguro M, Koike M, Yokoo Y, Suwa Y. Structure-activity relationship study on alpha1 adrenergic receptor antagonists from beer. Bioorg Med Chem Lett. 19 5905-8 (2009)

2008

  1. 123) Nagaoka Y, Ahmed M, Hossain M, Bhuiyan MA, Ishiguro M, Nakamura T, Watanabe M, Nagatomo T. Amino acids of the human alpha1d-adrenergic receptor involved in antagonist binding. J Pharmacol Sci. 106 114-120 (2008)

  2. 122) Ahmed M, Hossain M, Bhuiyan MA, Ishiguro M, Tanaka T, Muramatsu I, Nagatomo T., Mutational analysis of the alpha 1a-adrenergic receptor binding pocket of antagonists by radioligand binding assay. Biol Pharm Bull. 31 598-601 (2008)

  3. 121) Shimamura T, Hiraki K, Takahashi N, Hori T, Ago H, Masuda K, Takio K, Ishiguro M, Miyano M. Crystal structure of squid rhodopsin with intracellularly extended cytoplasmic region. J Biol Chem. 283, 17753-17756 (2008)

  4. 120) Wang F, Ishiguro M, Mutsukado M, Fujita K, Tanaka T., Microstructure of poly(gamma-glutamic acid) produced by Bacillus subtilis consisting of clusters of D- and L-glutamic acid repeating units. J Agric Food Chem. 56 4225-4228 (2008)

  5. 119) Hossain M, Ahmed M, Bhuiyan MA, Ishiguro M, Nakamura T, Ozaki M, Nagatomo T. Mutation of important amino acid residue of Asp104Lys in human beta(1)-adrenergic receptor triggers functional and constitutive inactivation. Biol Pharm Bull. 31 1517-1522 (2008)

  6. 118) Suzuki T, Igari S, Hirasawa A, Hata M, Ishiguro M, Fujieda H, Itoh Y, Hirano T, Nakagawa H, Ogura M, Makishima M, Tsujimoto G, Miyata N. Identification of G protein-coupled receptor 120-selective agonists derived from PPARgamma agonists. J Med Chem. 51 7640-4 (2008)

2007

  1. 117) Kasahara, T.; Maeda, M.; Ishiguro, M.; Kasahara, M., Identification by Comprehensive Chimeric Analysis of a Key Residue Responsible for High Affinity Glucose Transport by Yeast HXT2. J. Biol. Chem. 282 13146-13150 (2007).

  2. 116) Ito, K.; Asakura, T., Morita, Y; Nakajima, K.; Koizumi, A.; Shimizu-Ibuka, A.; Masuda, K.; Ishiguro, M.; Terada, T.; Maruyama, J.; Kitamoto, K.; Misaka, T.; Abe, K., Microbial production of sensory-active miraculin. Biochem. Biophys. Res. Comm. 360 407-411(2007)

  3. 115) Akuzawa, N.; Takeda, S.; Ishiguro, M., Structural modelling and mutation analysis of a nociceptin receptor and its ligand complexes. J. Biochem. 141 907-916 (2007)

2006

  1. 114) M. Ahmed, M. Ishiguro, T. Nagatomo, Molecular modeling of SWR-0342SA, a β3-selective agonist, with β1- and β3-adrenoceptor. Life Sci. 78 2019-2023 (2006).

  2. 113) M. Horikawa, K. Tateda, E. Tuzuki, Y. Ishii, C. Ueda, T. Takabatake, S. Miyairi, K. Yamaguchi, M. Ishiguro, Synthesis of Pseudomonas quorum-sensing autoinducer analogs and structural entities required for induction of apoptosis in macrophages. Bioorg. Med. Chem. Lett. 16 2130-2133 (2006).

  3. 112) J. F. Ma, K. Tamai, N. Yamaji, N. Mitani, S. Konishi, M. Katsuhara, M. Ishiguro, Y. Murata, M. Yano. A silicon transporter in rice. Nature 440 688-691 (2006).

  4. 111) K. Aoki, H. Saito, C. Itzstein, M. Ishiguro, T. Shibata, R. Blanque, A. Mian, H. Anower M. Takahashi, Y. Suzuki, M. Yoshimatsu, A. Yamaguchi, P. Deprez, P. Mollat, R. Murali, K. Ohya, W. Horne, R. Baron, A TNF receptor loop peptide mimic blocks RANK ligand-induced signaling, bone resorption, and bone loss. J Clinical Investigation 116 1525-1534 (2006).

  5. 110) T. Kasahara, M. Ishiguro, M. Kasahara, Eight Amino Acid Residues in Transmembrane Segments of Yeast Glucose Transporter Hxt2 Are Required for High Affinity Transport. J Biol. Chem. 281 18532-18538 (2006).

  6. 109) T. Shiba, S. Kakuda, M. Ishiguro, I. Morita, S. Oka, T. Kawasaki, S. Wakatsuki, R. Kato, Crystal structure of GlcAT-S, a human glucuronyltransferase, involved in the biosynthesis of the HNK-1 carbohydrate epitope. Proteins 65 499-508 (2006).

  7. 108) S. Miyairi, T. Tateda, E. T. Fuse, C. Ueda, H. Saito, T. Takabatake, Y. Ishii, M. Horikawa, M. Ishiguro, T. J. Standiford, K. Yamaguchi, Immunization with 3-oxododecanoyl-L-homoserine lactone-protein conjugate protects mice from lethal Pseudomonas aeruginosa lung infection. J. Medical Microbiiol. 55 1381-1387 (2006).

  8. 107) M. Ahmed, H. A. Muntasir, M. Hossain, M. Ishiguro, T. Komiyama, I. Muramatsu, H. Kurose, T. Nagatomo, Beta-Blockers Show Inverse Agonism to a Novel Constitutively Active Mutant of β1-Adrenoceptor. J. Phamacol Sci. 102 167-172 (2006).

  9. 106) H. A. Muntasir, H. A. Bhuiyan, M. Ishiguro, M. Ozaki, T. Nagatomo., Inverse Agonist Activity of Sarpogrelate, a Selective 5-HT2A-Receptor Antagonist, at the Constitutively Active Human 5-HT2A Receptor. J. Pharmacol. Sci. 102 189-195 (2006)

  10. 105) M. Ahmed, H. A. Muntasir, M. Ishiguro, T. Komiyama, I. Muramatsu, M. Hossain, T. Nakamura, T. Nagatomo, Pharmacological and Molecular Characterization of Novel SWR-Compounds for β-Adrenergic Receptors as assessed by Molecular Modeling, Site-Directed Mutagenesis, Binding Affinity and Functional Activity. Oyo Yakuri/Pharmacometrics 71 1-10 (2006).

2005

  1. 104) K. Masuda, K. Kawahara, A. Kuniyasu, M. Ishiguro, H. Nakayama, Stoichiometry and binding site analysis of ligand-human serum albumin complex by photoaffinity labeling and mass spectrometry. J. Mass Spec. Soc. Jpn. 53 18-24 (2005),

  2. 103) E. Tsuzuki, K. Tateda, S. Kimura, T. Matsumoto, Y. Ishii, S. Miyairi, M. Horikawa, M. Ishiguro, K. Yamaguchi, Examination on IL-8 production and apoptosis induction by autoinducers and their derivatives of Pseudomonas aeruginosa. Japanese J. Antibiot. 58(suppl. A) 100-103 (2005),

  3. 102) L. Ma, J. Alba, F. Y. Chang, M. Ishiguro, K. Yamaguchi, L. K. Siu, Y. Ishii, Novel SHV-derived extended-spectrum beta-lactamase, SHV-57, that confers resistance to ceftazidime but not cefazolin. Antimicrob. Agents Chemother. 49 600-605 (2005).

  4. 101) S. Shiraga, M. Ishiguro, H. Fukami, M. Nakao, M. Ueda, M., Creation of Rhizopus oryzae lipase having a unique oxyanion hole by computational mutagenesis in the lid domain. Appl. Microbiol. Biotechnol. 68, 779-785 (2005).

  5. 100) S. Shiraga, M. Kawakami, M. Ishiguro, M. Ueda, Enhanced Reactivity of Rhizopus oryzae Lipase Displayed on Yeast Cell Surfaces in Organic Solvents: Potential as a Whole-Cell Biocatalyst in Organic Solvents. Appl. Environment. Microbiol. 71 4335–4338 (2005).

  6. 99) K. Tsuchihara, K. Fujikawa, M. Ishiguro, T. Yamada, C. Tada, K. Ozaki, M. Ozaki, An Odorant-binding Protein Facilitates Odorant Transfer from Air to Hydrophilic Surroundings in the Blowfly. Chem. Senses 30 559-564 (2005).

  7. 98) N. Tamura, S. Murakami, Y. Oyama, M. Ishiguro, A. Yamaguchi, Direct Interaction of Multidrug Efflux Transporter AcrB and Outer Membrane Channel TolC Detected via Site-Directed Disulfide Cross-Linking. Biochemistry 44 11115-11121 (2005).

  8. 97) L. Ma, J. Alba, F.-L. Chang, M. Ishiguro, K. Yamaguchi, L. K. Siu, Y. Ishii, Novel SHV-Derived Exttebded-Spectrum b-Lactamase, SHV-57, that confers Resistance to Ceftazidime but not Cefazolin. Antimicrob. Agents Chemother. 49, 600-605 (2005).

2004

  1. 96) Y. Doi, Y. Wachino, M. Ishiguro, H. Kurokawa, K. Yamane, N. Shibata, K. Shibayama, K. Yokoyama, H. Kato, T. Yagi, Y. Arakawa, Y. Characterization of an Inhibitor-Sensitive AmpC β-Lactamase Variant Produced by an Escherichia coli Clinical Isolate Resistant to Oxyiminocephalosporins and Cephamycins. Antimicrob. Agents Chemother. 48, 2652-2658 (2004).

  2. 95) M. Ishiguro, Y. Oyama, T. Hirano, Structural Models of the Photointermediates in the Rhodopsin Photocascade, Lumirhodopsin, Metarhodopsin I, and Metarhodopsin II. ChemBioChem. 5, 298-310, (2004).

  3. 94) S. Kimura, M. Ishiguro, Y. Ishii, J. Alba, K. Yamaguchi, Role of a Mutation at Position 167 of CTX-M-19 in Ceftazidime Hydrolysis. Antimicrob. Agents Chemother. 48, 1454-1460 (2004).

  4. 93) K. Sugase, M. Horikawa, M. Sugiyama, M. Ishiguro, Restriction of a Peptide Turn Conformation and Conformational Analysis of Guanidino Group using Arginine-Proline Fused Amino Acids: Application to Mini Atrial Natriuretic Peptide on Binding to the Receptor. J. Med. Chem. 47, 489-492 (2004).

  5. 92) S. Kakuda, T. Shiba, T., M. Ishiguro, H. Tagawa, S. Oka, Y. Kajiwara, T. Kawasaki, S. Wakatsuki, R. Kato, Structural Basis for Acceptor Substrate Recognition of a Human Glucuronyltransferase, GlcAT-P, Which is Involved in the Biosynthesis of the Carbohydrate Epitope HNK-1. J. Biol. Chem. 279 22693 – 22703 (2004).

  6. 91) T. Kasahara, M. Ishiguro, M. Kasahara, Comprehensive Chimeric Analysis of Amino Acid Residues Critical for High-affinity Glucose Transport by Hxt2 of Saccharomyces cerevisiae. J. Biol. Chem. 279 30274-30278 (2004).

  7. 90) M. Ishiguro, Conformational Analysis of Glu181 and Ser186 in the Metarhodopsin I State. ChemBioChem 5 1204-1209 (2004).

  8. 89) M. Ishiguro, Ligand-binding Modes in Cationic Biogenic Amine Receptors, ChemBioChem 5 1210-1219 (2004).

  9. 88) K. Yamamoto, H. Masuno, N. Sawada, T. Sakaki, K. Inouye, M. Ishiguro, S. Yamada, Homology Modeling of Human 25-Hydroxyvitamin D3 1α-Hydroxylase (CYP27B1) based on the Crystal Structure of Rabbit CYP2C5. J. Steroid Biochem. Mol. Biol. 89-90, 167-171 (2004).

  10. 87) A. Inano, Y. Sai, Y. Kato, I. Tamai, M. Ishiguro, A. Tsuji, Functional regions of organic cation/carnitine transporter OCTN2 (SLC22A5): Roles in carnitine recognition. Drug Metabolism and Pharmacokinetics19 180-189 (2004)

2003

  1. 86) K. Tateda, Y. Ishii, M. Horikawa, T. Matsumoto, S. Miyairi, J. C. Pechere, T. Standiford, M. Ishiguro, K. yamaguchi, The Pseudomonas aeruginosa Autoinducer N-3-Oxododecanoyl Homoserine Lactone Accelerates Apooptosis in Macrophages and Neutrophils. Infection and Immunity, 71 5785-5793 (2003).

  2. 85) M. Ishiguro, T. Hirano, Y. Oyama, Modeling of Photointermediates Suggests a Mechanism of the Flip of the β-Ionone Moiety of the Retinylidene Chromophore in the Rhodopsin Photocascade. ChemBioChem 4 228-231 (2003).

  3. 84) Y. Kato-Toma, T. Iwashita, K. Matsuda, Y. Oyama, and M. Ishiguro, pKa Measurements from Nuclear Magnetic Resonance of Tyrosine-150 in Class C β-Lactamase. Biochem. J. 371 175-181 (2003).

  4. 83) M. Rashid, P. Manivet, H. Nishino, J. Pratuangdejkul, M. Rajab, M. Ishiguro, J.-M. Launay, T. Nagatomo, Identification of the Binding Sites and Selectivity of Sarpogrelate, a Novel 5-HT2 Antagonist, to Human 5-HT2A, 5-HT2B and 5-HT2C Receptor Subtypes by Molecular Modeling. Life Sci. 73, 193-207 (2003).

  5. 82) A. Shimizu-Ibuka, Y. Ishii, M. Galleni, M. Ishiguro, K. Yamaguchi, J.-M. Frere, H. Matsuzawa, H. Sakai, Crystal Structure of Extended-spectrum β-Lactamase Toho-1: Insights into the Molecular Mechanism for Catalytic Reaction and Substrate Specificity Expansion. Biochemistry 42, 10634-10643 (2003).

  6. 81) J. Alba, C. Bauvois, Y. Ishii, M. Galleni, K. Matsuda, M. Ishiguro, M. Ito, J.-M. Frere, K. Yamaguchi, A detailed Kinetic study of Mox-1, a Plasmid-encoded Class C β-Lactamase. FEMS Microbiol. Lett. 225, 183-188 (2003).

2002

  1. 80) K. Sugase, Y. Oyama, K. Kitano, T. Iwashita, T. Fujiwara, H. Akutsu, and M. Ishiguro, Designing Analogs of Mini Atrial Natriuretic Peptide Based on Structural Analysis by NMR and Restrained Molecular Dynamics. J. Med. Chem. 45, 881-887 (2002).

  2. 79) K. Sugase, Y. Oyama, K. Kitano, H. Akutsu, and M. Ishiguro, Structure-Activity Relationships for Mini Atrial Natriuretic Peptide by Proline Scanning Mutagenesis and Shortening of Peptide Backbone. Bioorg. Med. Chem. Lett. 12 1245-1247 (2002).

  3. 78) K. Kawahara, A. Kuniyasu, K. Masuda, M. Ishiguro, and H. Nakayama, Efficient Identification of Photolabeled Amino Acid Resisues by Combining Immunoaffinity Purification with Mass Spectrometry. Biochem. J. 363 223-232 (2002).

  4. 77) H. Furukawa, T. Hamada, M.K. Hayashi, T. Haga, Y. Muto, H. Hirota, S. Yokoyama, K. Nagasawa, and M. Ishiguro, Conformation of Ligands Bound to the Muscarinic Acetylcholine Receptor. Mol. Pharmacol. 62 778-787 (2002).

  5. 76) B. Indra, K. Matsunaga, O. Hoshino, M. Suzuki, H. Ogasawara, M. Ishiguro, and Y. Ohizumi, Structure-activity relationship on (±)-nantenine derivatives in antiserotonergic activities in rat aorta. Can. J. Physiol. Pharmacol. 80 198-204 (2002).

  6. 75) T. Shimamura, A. Ibuka, S. Fushinobu, T. Wakagi, M. Ishiguro, Y. Ishii, and H. Matsuzawa, Acyl-Intermediate Structures of the Extended-Spectrum Class A β-Lactamase, Toho-1, in Complex with Cefotaxime, Cephalothin, and Benzylpenicillin. J. Biol. Chem. 277 46601-46608 (2002).

  7. 74) T. Hirano, I.-T. Lim, D-M. Kim, X-G. Zheng K. Yoshihara, Y. Oyama, H. Imai, Y. Shichida and M. Ishiguro, Constraints of Opsin Structure on the Ligand Binding Site: Studies with Ring-fused Retinals. Photochem. Photobiol. 76 606-615 (2002).

  8. 73) M. Ishiguro, Y. Futabayashi, T. Ohnuki, M. Ahmed, I. Muramatsu, and T. Nagatomo, Identification of Binding Sites of Prazosin, Tamuslosin and KMD-3213 with α1-Adrenergic Receptor Subtypes by Molecular Modeling. Life Sci. 71 2531-2541 (2002).

2001

  1. 72) Y. Kan, D. Uemura, Y. Hirata, M. Ishiguro, and T. Iwashita. Complete NMR Signal Assignment of Palytoxin and N-Acetylpalytoxin. Tet. Lett. 42, 3197-3202 (2001).

  2. 71) Y. Hatanaka, M. Ishiguro, M. Hashimoto, L. N. Gastinel and K. Nakagomi. A Model of Photoprobe Docking with β1,4-Galactosyltransferase Identifies a Possible Carboxylate Involved in Glycosylation Steps. Bioorg. Med. Chem. Lett. 11, 411-413 (2001).

  3. 70) Y. Kato-Toma, and M. Ishiguro. Reaction of Lys-Tyr-Lys Triad Mmimics with Benzylpenicillin: Insight into the Role of Tyr 150 in Class C β-Lactamases. Bioorg. Med. Chem. Lett. 11, 1161-1164 (2001).

  4. 69) T. Nagatomo, T. Ohnuki, M. Ishiguro, M. Ahmed, and T. Nakamura, β-Adrenoceptors: Three-Dimensional Structures and Binding Sites for Ligands. Japanese J. Pharmacol. 87, 7-13 (2001).

2000

  1. 68) M. Ishiguro, A Mechanism of Primary Photoactivation Reactions of Rhodopsin: Modeling of the Intermediates in the Rhodopsin Photocycle. J. Am. Chem. Soc. 122 444-451 (2000).

  2. 67) M. Hirama, K. Akiyama, T. Tanaka, T. Noda, K. Iida, I. Sato, R. Hanaishi, S.Fukuda-Ishisaka, M. Ishiguro, Y. Otani, and J.E. Leet. Paramagnetic Enediyne Antibiotic C-1027: Spin Identification and Characterization of Radical Species. J. Am. Chem. Soc.122, 720-721 (2000).

  3. 66) T. Kawano, Y. Ito, M. Ishiguro, K. Takuwa, T. Nakajima, and Y. Kimura. Molecular Cloning and Characterization of a New Insulin/IGF-like Peptide of the Nematode Caenorhabditis elegans. Biochem. Biophys. Res. Commun. 273, 431-436 (2000).

  4. 65) K. Ohtsubo, S. Imajo, M. Ishiguro, T. Nakatani, S. Oka, and T. Kawasaki, Studies on the Structure-Function Relationship of the HNK-1 Associated Glucuronyltransferase, GlcAT-P, by Computer Modeling and Site-Directed Mutagenesis. J. Biochem. 128, 283-291 (2000).

学術論文: 宮崎達雄

  1. 1) Enzymic syntheses of GlcNAcb1-2Man and Galb1-4GlcNAcb1-2Man as components of complex type sugar chains; Fujimoto, Hiroshi; Isomura, Megumi; Miyazaki, Tatsuo; Matsuo, Ichiro; Walton, Richard; Sakakibara, Tohru; Ajisaka, Katsumi, Glycoconjugate Journal (1997), 14(1), 75-80.

  2. 2) Chemoenzymic synthesis of the branched oligosaccharides which correspond to the core structures of N-linked sugar chains; Matsuo, Ichiro; Isomura, Megumi; Miyazaki, Tatsuo; Sakakibara, Tohru; Ajisaka, Katsumi, Carbohydrate Research (1998), 305(3-4), 401-413.

  3. 3) A short, efficient synthesis of the octamannan residue of high mannose type sugar chains; Matsuo, Ichiro; Miyazaki, Tatsuo; Isomura, Megumi; Sakakibara, Tohru; Ajisaka, Katsumi, Journal of Carbohydrate Chemistry (1998), 17(8), 1249-1258.

  4. 4) Chemoenzymic Synthesis of the 9-Deoxy-9-fluoro-[3-13C]-NeuAc-a-(2®6)-[U-13C]-b-Gal Sequence on an Intact; Glycoprotein, Miyazaki, Tatsuo; Sakakibara, Tohru; Sato, Hajime; Kajihara, Yasuhiro, Journal of the American Chemical Society (1999), 121(6), 1411-1412.

  5. 5) Synthesis of regioselectively protected forms of cytidine based on enzyme-catalyzed deacetylation as the key step; Kuboki, Atsuhito; Ishihara, Takashi; Kobayashi, Eiko; Ohta, Hiromichi; Ishii, Takeshi; Inoue, Ayumu; Mitsuda,Satoshi; Miyazaki, Tatsuo; Kajihara, Yasuhiro; Sugai, Takeshi, Bioscience, Biotechnology, and Biochemistry (2000), 64(2), 363-368.

  6. 6) An Approach to the Precise Chemoenzymatic Synthesis of 13C-Labeled Sialyloligosaccharide on an Intact Glycoprotein: A Novel One-Pot [3-13C]-Labeling Method for Sialic Acid Analogues by Control of the Reversible Aldolase Reaction, Enzymatic Synthesis of [3-13C]-NeuAc-a-(2®3)-[U-13C]-Gal-b-(1®4)-GlcNAc-b- Sequence onto Glycoprotein, and Its Conformational Analysis by Developed NMR Techniques, Miyazaki, Tatsuo; Sato, Hajime; Sakakibara, Tohru; Kajihara, Yasuhiro, Journal of the American Chemical Society (2000), 122(24), 5678-5694.

  7. 7) Synthesis of diverse asparagine linked oligosaccharides and synthesis of sialylglycopeptide on solid phase, Kajihara Yasuhiro; Yamamoto Naoki; Miyazaki Tatsuo; Sato Hajime, Current medicinal chemistry (2005), 12(5), 527-50.

  8. 8) Synthesis of CMP-9''-modified-sialic acids as donor substrate analogues for mammalian and bacterial sialyltransferases, Kajihara, Yasuhiro; Kamitani, Toshimi; Sato, Reiko; Kamei, Naoki; Miyazaki, Tatsuo; Okamoto, Ryo; Sakakibara, Thoru; Tsuji, Takashi; Yamamoto, Takeshi, Carbohydrate Research (2007), 342(12-13), 1680-1688.

  9. 9) Syntheses and glycosidation reactions of 6-thiosialosyl donors, Yuasa, Hideya; Tanetani, Yoshitaka; Miyazaki, Tatsuo; Izumi, Masayuki; Hashimoto, Hironobu, Letters in Organic Chemistry (2008), 5(4), 262-264.

  10. 10) Stereoselective synthesis of 5-thio-b-L-fucopyranosyl phosphate, Miyazaki, Tatsuo; Hiyoshi, Daisuke; Izumi, Masayuki; Hashimoto, Hironobu; Yuasa, Hideya, From Letters in Organic Chemistry (2008), 5(7), 576-578.

  11. 11) CMP substitutions preferentially inhibit polysialic acid synthesis, Miyazaki, Tatsuo; Angata, Kiyohiko; Seeberger, Peter H.; Hindsgaul, Ole; Fukuda, Minoru, Glycobiology (2008), 18(2), 187-194.

  12. 12) The N-glycolyl form of mouse sialyl Lewis X is recognized by selectins but not by HECA-452 and FH6 antibodies that were raised against human cells, Mitoma, Junya; Miyazaki, Tatsuo; Sutton-Smith, Mark; Suzuki, Misa; Saito, Hideo; Yeh, Jiunn-Chern; Kawano, Takehiro; Hindsgaul, Ole; Seeberger, Peter H.; Panico, Maria; Glycoconjugate Journal (2009), 26(5), 511-523.

  13. 13) Evaluation and Comparison of the Antioxidative Potency of Various Carbohydrates Using Different Methods, Ajisaka, Katsumi; Agawa, Sayuri; Nagumo, Satomi; Kurato, Keisuke; Yokoyama, Tatsuya; Arai, Kumiko; Miyazaki, Tatsuo, Journal of Agricultural and Food Chemistry (2009), 57(8), 3102-3107.

  14. 14) Enzymatic synthesis of unique sialyloligosaccharides using marine bacterial a-(2®3)- and a-(2®6)-sialyltransferases, Mine, Toshiki; Miyazaki, Tatsuo; Kajiwara, Hitomi; Naito, Kenta; Ajisaka, Katsumi; Yamamoto, Takeshi, Carbohydrate Research (2010), 345(10), 1417-1421.

  15. 15) A recombinant a-(2®3)-sialyltransferase with an extremely broad acceptor substrate specificity from Photobacterium sp. JT-ISH-224 can transfer N-acetylneuraminic acid to inositols, Mine, Toshiki; Miyazaki, Tatsuo; Kajiwara, Hitomi; Tateda, Naoya; Ajisaka, Katsumi; Yamamoto, Takeshi, Carbohydrate Research (2010), 345(17), 2485-2490.

  16. 16) A CMP-N-acetylneuraminic acid synthetase purified from a marine bacterium, Photobacterium leiognathi JT-SHIZ-145, Kajiwara, Hitomi; Mine, Toshiki; Miyazaki, Tatsuo; Yamamoto, Takeshi, Bioscience, Biotechnology, and Biochemistry (2011), 75(1), 47-53.

  17. 17) A novel two-step synthesis of a-linked mannobioses based on an acid-assisted reverse hydrolysis reaction, Ajisaka, Katsumi; Yagura, Misato; Miyazaki, Tatsuo, Carbohydrate Research (2012), 347(1), 147-150.

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