Niigata University of Pharmacy and Applied Life Sciences
Laboratory for Gene Regulation Study

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Research

• What we are doing

Members

• Assistant professor: Mitsuoki Kawano

Projects

• Development of a strategy for site-specific RNA cleavage in E. coli based on TRUE gene silencing
• Analysis of novel functional RNAs in germ cells
• Analysis of RNA editing sites on pre-miRNAs

Publications

• Small Untranslated RNA

Small Untranslated RNA

Asterisk (*) shows a corresponding author and underline shows a first or joint first author.

1. Kawano M*, Divergently overlapping cis-encoded antisense RNA regulating toxin-antitoxin systems from E. coli: hok/sok, ldr/rdl, symE/symR. RNA Biology. 2012; in press.
2. Kawano M*, Kawaji H, Grandjean V, Kiani J, Rassoulzadegan M. Novel Small noncoding RNAs in Mouse Spermatozoa, Zygotes and Early Embryos. PLoS One. 2012;7(9):e44542.
3. AndoY, Burroughs A, Kawano M, M., de Hoon, M. J., and Hayashizaki, Y. Targeted Methods to Improve Small RNA Profiles Generated by Deep Sequencing. Regulatory RNAs: Basics and Applications. 2012;253-72.
4. Burroughs AM, Kawano M*, Ando Y, Daub CO, Hayashizaki Y. pre-miRNA profiles obtained through application of locked nucleic acids and deep sequencing reveals complex 5'/3' arm variation including concomitant cleavage and polyuridylation patterns. Nucleic Acids Res. 2012 Feb;40(4):1424-37.
5. Shinohara Y, Yahagi K, Kawano M, Nishiyori H, Kawazu C, Suzuki N, Manabe R, Hirase H. miRNA profiling of bilateral rat hippocampal CA3 by deep sequencing. Biochem Biophys Res Commun. 2011 Jun 3;409(2):293-8.
6. Kawano M*, Kawazu C, Lizio M, Kawaji H, Carninci P, Suzuki H, Hayashizaki Y. Efficient small RNA library preparation protocol. Biotechniques Protocol Guide. 2011;4-6.
7. Kawano M*, Kawazu C, Lizio M, Kawaji H, Carninci P, Suzuki H, Hayashizaki Y. Reduction of non-insert sequence reads by dimer eliminator LNA oligonucleotide for small RNA deep sequencing. Biotechniques. 2010 Oct;49(4):751-5.
8. de Hoon MJ, Taft RJ, Hashimoto T, Kanamori-Katayama M, Kawaji H, Kawano M, Kishima M, Lassmann T, Faulkner GJ, Mattick JS, Daub CO, Carninci P, Kawai J, Suzuki H, Hayashizaki Y. Cross-mapping and the identification of editing sites in mature microRNAs in high-throughput sequencing libraries. Genome Res. 2010 Feb;20(2):257-64.
9. Fozo EM, Kawano M, Fontaine F, Kaya Y, Mendieta KS, Jones KL, Ocampo A, Rudd KE, Storz G. Repression of small toxic protein synthesis by the Sib and OhsC small RNAs. Mol Microbiol. 2008 Dec;70(5):1076-93.
10. Kawano M, Aravind L, Storz G. An antisense RNA controls synthesis of an SOS-induced toxin evolved from an antitoxin. Mol Microbiol. 2007 May;64(3):738-54.
11. Kanie S, Horibata K, Kawano M, Isogawa A, Sakai A, Matsuo N, Nakanishi M, Hasegawa K, Yoshiyama K, Maki H. Roles of RecA protein in spontaneous mutagenesis in Escherichia coli. Genes Genet Syst. 2007 Apr;82(2):99-108.
12. Kawano M, Storz G, Rao BS, Rosner JL, Martin RG. Detection of low-level promoter activity within open reading frame sequences of Escherichia coli. Nucleic Acids Res. 2005 Oct 31;33(19):6268-76.
13. Kawano M, Reynolds AA, Miranda-Rios J, Storz G. Detection of 5'- and 3'-UTR-derived small RNAs and cis-encoded antisense RNAs in Escherichia coli. Nucleic Acids Res. 2005 Feb 17;33(3):1040-50.
14. Kawano M, Oshima T, Kasai H, Mori H. Molecular characterization of long direct repeat (LDR) sequences expressing a stable mRNA encoding for a 35-amino-acid cell-killing peptide and a cis-encoded small antisense RNA in Escherichia coli. Mol Microbiol. 2002 Jul;45(2):333-49.
15. Kawano M, Kanaya S, Oshima T, Masuda Y, Ara T, Mori H. Distribution of repetitive sequences on the leading and lagging strands of the Escherichia coli genome: comparative study of Long Direct Repeat (LDR) sequences. DNA Res. 2002 Feb 28;9(1):1-10.