原著論文
Muhs M, Yamamoto H, Ismer J, Takaku H, Nashimoto M, Uchiumi T, Nakashima N, Mielke T, Hildebrand PW, Nierhaus KH, Spahn CM.
“Structural basis for the binding of IRES RNAs to the head of the ribosomal 40S subunit.”
Nucleic Acids Res.2011 39(12):5264-75.
Elbarbary RA*, Takaku H*, Uchiumi N, Tamiya H, Abe M, Nishida H, Nashimoto M.
“Human cytosolic tRNase ZL can downregulate gene expression through miRNA.”
FEBS Lett.2009 583(19):3241-6 (*contributed equal to this work).
Elbarbary RA*, Takaku H*, Uchiumi N, Tamiya H, Abe M, Takahashi M, Nishida H, Nashimoto M.
“Modulation of gene expression by human cytosolic tRNase Z(L) through 5'-half-tRNA.”
PLoS One.2009 4(6):e5908 (*contributed equal to this work)
Elbarbary RA, Takaku H, Tamura M, Nashimoto M.
“Inhibition of vascular endothelial growth factor expression by TRUE gene silencing.”
Biochem Biophys Res Commun.2009 379(4):924-7.
Takaku H, Nashimoto M.
“Escherichia coli tRNase Z can shut down growth probably by removing amino acids from aminoacyl-tRNAs.”
Genes Cells.2008 13(11):1087-97.
Elbarbary RA*, Takaku H*, Nashimoto M.
“Functional analyses for tRNase Z variants: an aspartate and a histidine in the active site are essential for the catalytic activity.”
Biochim Biophys Acta.2008 1784(12):2079-85. (*contributed equal to this work)
Minagawa A, Ishii R, Takaku H, Yokoyama S, Nashimoto M.
“The flexible arm of tRNase Z is not essential for pre-tRNA binding but affects cleavage site selection.”
J Mol Biol.2008 381(2):289-99.
Takahashi M, Takaku H, Nashimoto M.
“Regulation of the human tRNase ZS gene expression.”
FEBS Lett.2008 582(17):2532-6.
Ishii R, Minagawa A, Takaku H, Takagi M, Nashimoto M, Yokoyama S.
“The structure of the flexible arm of Thermotoga maritima tRNase Z differs from those of homologous enzymes.”
Acta Crystallogr Sect F Struct Biol Cryst Commun.2007 63(Pt 8):637-41.
Kogure T, Wakisaka N, Takaku H, Takagi M.
“Efficient production of 2-deoxy-scyllo-inosose from d-glucose by metabolically engineered recombinant Escherichia coli.”
J Biotechnol.2007 129(3):502-9.
Takaku H, Kimoto A, Kodaira S, Nashimoto M, Takagi M.
“Isolation of a Gram-positive poly(3-hydroxybutyrate) (PHB)-degrading bacterium from compost, and cloning and characterization of a gene encoding PHB depolymerase of Bacillus megaterium N-18-25-9.”
FEMS Microbiol Lett.2006 264(2):152-9.
Minagawa A, Takaku H, Ishii R, Takagi M, Yokoyama S, Nashimoto M.
“Identification by Mn2+ rescue of two residues essential for the proton transfer of tRNase Z catalysis.”
Nucleic Acids Res.2006 34(13):3811-8.
Nakashima A, Takaku H, Shibata HS, Negishi Y, Takagi M, Tamura M, Nashimoto M.
“Gene silencing by the tRNA maturase tRNase ZL under the direction of small-guide RNA.”
Gene Ther.2007 14(1):78-85.
Minagawa A*, Takaku H*, Shibata HS, Ishii R, Takagi M, Yokoyama S, Nashimoto M.
“Substrate recognition ability differs among various prokaryotic tRNase Zs.”
Biochem Biophys Res Commun.2006 345(1):385-93. (*contributed equal to this work)
Shibata HS, Minagawa A, Takaku H, Takagi M, Nashimoto M.
“Unstructured RNA is a substrate for tRNase Z.”
Biochemistry.2006 45(17):5486-92.
Takaku H, Kodaira S, Kimoto A, Nashimoto M, Takagi M.
“Microbial communities in the garbage composting with rice hull as an amendment revealed by culture-dependent and -independent approaches.”
J Biosci Bioeng.2006 101(1):42-50.
Minagawa A, Takaku H, Takagi M, Nashimoto M.
“The missense mutations in the candidate prostate cancer gene ELAC2 do not alter enzymatic properties of its product.”
Cancer Lett.2005 222(2):211-5.
Shibata HS, Takaku H, Takagi M, Nashimoto M.
“The T loop structure is dispensable for substrate recognition by tRNase ZL.”
J Biol Chem.2005 280(23):22326-34.
Ishii R, Minagawa A, Takaku H, Takagi M, Nashimoto M, Yokoyama S.
“Crystal structure of the tRNA 3' processing endoribonuclease tRNase Z from Thermotoga maritima.”
J Biol Chem.2005 280(14):14138-44.
Habu Y, Miyano-Kurosaki N, Kitano M, Endo Y, Yukita M, Ohira S, Takaku H, Nashimoto M, Takaku H.
“Inhibition of HIV-1 gene expression by retroviral vector-mediated small-guide RNAs that direct specific RNA cleavage by tRNase ZL.”
Nucleic Acids Res.2005 33(1):235-43.
Takaku H, Minagawa A, Takagi M, Nashimoto M.
“The N-terminal half-domain of the long form of tRNase Z is required for the RNase 65 activity.”
Nucleic Acids Res.200432(15):4429-38.
Takaku H, Minagawa A, Takagi M, Nashimoto M.
“A novel 4-base-recognizing RNA cutter that can remove the single 3' terminal nucleotides from RNA molecules.”
Nucleic Acids Res.2004 32(11):e91.
Takaku H, Mutoh E, Sagehashi Y, Fukuda R, Horiuchi H, Ochi K, Takagi M, Ohta A.
“A Gcn4p homolog is essential for the induction of a ribosomal protein L41 variant responsible for cycloheximide resistance in the yeast Candida maltosa.”
J Biol Chem.2004 279(22):23030-7.
Minagawa A*, Takaku H*, Takagi M, Nashimoto M.
“A novel endonucleolytic mechanism to generate the CCA 3' termini of tRNA molecules in Thermotoga maritima.”
J Biol Chem.2004 279(15):15688-97. (*contributed equal to this work)
Takaku, H., Minagawa, A., Takagi, M., Nashimoto, M.
“A candidate prostate cancer susceptibility gene encodes tRNA 3' processing endoribonuclease.”
Nucleic Acids Res.31(9):2272-8 (2003)
Takaku H, Horiuchi H, Takagi M, Ohta A.
“Pseudohyphal growth in a dimorphic yeast, Candida maltosa, after disruption of the C-GCN4 gene, a homolog of Saccharomyces cerevisiae GCN4.”
Biosci. Biotechnol. Biochem.66(9):1936-9 (2002)
Takaku H, Mutoh E, Horiuchi H, Ohta A, Takagi M.
“Ray38p, a homolog of a purine motif triple-helical DNA-binding protein, Stm1p, is a ribosome-associated protein and dissociated from ribosomes prior to the induction of cycloheximide resistance in Candida maltosa.”
Biochem. Biophys. Res. Commun.284(1):194-202 (2001)
Mutoh E, Takaku H, Ohta A, Takagi M.
“A gene coding for a ribosomal protein L41 in cycloheximide-resistant ribosomes has a promoter which is upregulated under the growth-inhibitory conditions in yeast, Candida maltosa.”
Biochem. Biophys. Res. Commun.258(3):611-5 (1999)